(Associate Members) competition results from 2000/2001
A full listing of matches played in the competition throughout the season
| Southern Section 2nd Round |
| Tuesday, 9th January 2001 |
| Bournemouth (LD2) | 0 | Swansea City (LD2) | 1 | 3,810 |
| Brighton & Hove Albion (LD3) | 2 | Brentford (LD2) | 2 | 2,482 |
| after extra time, 90 minutes 2-2, Brentford won 4-2 on penalties |
| Bristol Rovers (LD2) | 3 | Plymouth Argyle (LD3) | 0 | 3,781 |
| Hereford United (CONF) | 1 | Reading (LD2) | 2 | 1,693 |
| Leyton Orient (LD3) | 0 | Wycombe Wanderers (LD2) | 2 | 946 |
| Millwall (LD2) | 0 | Swindon Town (LD2) | 0 | 2,394 |
| after extra time, 90 minutes 0-0, Swindon Town won 3-2 on penalties |
| Peterborough United (LD2) | 1 | Barnet (LD3) | 3 | 1,891 |
| Southend United (LD3) | 3 | Cambridge United (LD2) | 1 | 1,694 |
| Northern Section 2nd Round |
| Tuesday, 9th January 2001 |
| Chesterfield (LD3) | 4 | Macclesfield Town (LD3) | 1 | 1,839 |
| Halifax Town (LD3) | 2 | Stoke City (LD2) | 3 | 1,917 |
| Hartlepool United (LD3) | 3 | Doncaster Rovers (CONF) | 1 | 2,466 |
| Lincoln City (LD3) | 3 | Blackpool (LD3) | 1 | 962 |
| Wednesday, 10th January 2001 |
| Bury (LD2) | 2 | Kidderminster Harriers (LD3) | 0 | 1,015 |
| Tuesday, 16th January 2001 |
| Darlington (LD3) | 2 | Shrewsbury Town (LD3) | 0 | 1,502 |
| Tuesday, 30th January 2001 |
| Port Vale (LD2) | 2 | Chester City (CONF) | 0 | 2,507 |
| Walsall (LD2) | 2 | Wigan Athletic (LD2) | 1 | 3,436 |
| Southern Section 1st Round |
| Tuesday, 28th November 2000 |
| Barnet (LD3) | 2 | Rushden & Diamonds (CONF) | 0 | 887 |
| Tuesday, 5th December 2000 |
| Bournemouth (LD2) | 1 | Dover Athletic (CONF) | 1 | 2,171 |
| after extra time, 90 minutes 1-1, Bournemouth won 4-2 on penalties |
| Brentford (LD2) | 4 | Oxford United (LD2) | 1 | 1,517 |
| Brighton & Hove Albion (LD3) | 2 | Cardiff City (LD3) | 0 | 2,364 |
| Cambridge United (LD2) | 2 | Colchester United (LD2) | 0 | 1,555 |
| Millwall (LD2) | 4 | Northampton Town (LD2) | 1 | 2,369 |
| Peterborough United (LD2) | 1 | Luton Town (LD2) | 0 | 2,075 |
| Plymouth Argyle (LD3) | 3 | Bristol City (LD2) | 0 | 1,364 |
| Southend United (LD3) | 2 | Cheltenham Town (LD3) | 0 | 1,000 |
| Wycombe Wanderers (LD2) | 1 | Exeter City (LD3) | 0 | 1,022 |
| Saturday, 9th December 2000 |
| Torquay United (LD3) | 0 | Bristol Rovers (LD2) | 2 | 1,370 |
| Tuesday, 19th December 2000 |
| Hereford United (CONF) | 4 | Yeovil Town (CONF) | 0 | 853 |
| Northern Section 1st Round |
| Tuesday, 28th November 2000 |
| Lincoln City (LD3) | 3 | Morecambe (CONF) | 2 | 1,194 |
| Tuesday, 5th December 2000 |
| Chester City (CONF) | 1 | Hull City (LD3) | 0 | 770 |
| Doncaster Rovers (CONF) | 3 | Rochdale (LD3) | 2 | 1,453 |
| after extra time, 90 minutes 2-2, Doncaster Rovers won on golden goal rule |
| Rotherham United (LD2) | 3 | Chesterfield (LD3) | 4 | 3,488 |
| after extra time, 90 minutes 3-3, Chesterfield won on golden goal rule |
| Wednesday, 6th December 2000 |
| Stoke City (LD2) | 3 | Scarborough (CONF) | 0 | 2,336 |
| Saturday, 9th December 2000 |
| Bury (LD2) | 2 | Mansfield Town (LD3) | 1 | 1,117 |
| Wrexham (LD2) | 0 | Halifax Town (LD3) | 1 | 1,545 |
| Monday, 11th December 2000 |
| Hartlepool United (LD3) | 3 | Scunthorpe United (LD3) | 2 | 1,538 |
| Tuesday, 12th December 2000 |
| Kidderminster Harriers (LD3) | 2 | Carlisle United (LD3) | 1 | 777 |
| after extra time, 90 minutes 1-1, Kidderminster Harriers won on the golden goal rule |
| Tuesday, 9th January 2001 |
| Oldham Athletic (LD2) | 2 | Wigan Athletic (LD2) | 3 | 2,551 |
| after extra time, 90 minutes 2-2, Wigan Athletic won on golden goal rule |
| Port Vale (LD2) | 3 | Notts. County (LD2) | 0 | 1,919 |
| York City (LD3) | 0 | Darlington (LD3) | 4 | 1,095 |
